HSP70 expression in Biomphalaria glabrata snails exposed to cadmium

Rebeca da Silva Cantinha; Sueli Ivone Borrely; Nancy Oguiura; Carlos Alberto de Bragança Pereira; Marcela M Rigolon; Eliana Nakano

doi:10.1016/j.ecoenv.2017.02.026

HSP70 expression in Biomphalaria glabrata snails exposed to cadmium

Ecotoxicol Environ Saf. 2017 Jun:140:18-23. doi: 10.1016/j.ecoenv.2017.02.026. Epub 2017 Feb 27.

Authors

Rebeca da Silva Cantinha¹, Sueli Ivone Borrely², Nancy Oguiura³, Carlos Alberto de Bragança Pereira⁴, Marcela M Rigolon⁵, Eliana Nakano⁶

Affiliations

¹ Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil; Instituto de Pesquisas Energéticas e Nucleares, Avenida Professor Lineu Prestes, 2242, Cidade Universitária, São Paulo, SP CEP 05508-000, Brazil. Electronic address: rcantinha@gmail.com.
² Instituto de Pesquisas Energéticas e Nucleares, Avenida Professor Lineu Prestes, 2242, Cidade Universitária, São Paulo, SP CEP 05508-000, Brazil. Electronic address: sborrely@ipen.br.
³ Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil. Electronic address: nancyoguiura@butantan.gov.br.
⁴ Departamento de Estatística, Instituto de Matemática e Estatística, Universidade de São Paulo, Rua do Matão, 1010, Cidade Universitária, São Paulo, SP CEP 05008-090, Brazil. Electronic address: cadebp@gmail.com.
⁵ Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil. Electronic address: marcelarigolon@hotmail.com.
⁶ Instituto Butantan, Laboratório de Parasitologia, Avenida Vital Brasil, 1500, Butantã, São Paulo, SP CEP 05503-900, Brazil. Electronic address: eliana.nakano@butantan.gov.br.

PMID: 28231501
DOI: 10.1016/j.ecoenv.2017.02.026

Abstract

In this study, the effects of the heavy metal cadmium on the stress protein HSP70 are investigated in freshwater mollusks Biomphalaria glabrata. Adult snails were exposed for 96h to CdCl₂ at concentrations ranging from 0.09 to 0.7mgL^-1 (LC_50/96h=0.34 (0.30-0.37). Time and concentration-dependent increases in the expression of HSP70 were observed at sub-lethal levels in the immunoblotting assay. Further, an increased survival to a lethal heat shock was observed in animals pre-exposed to a nonlethal concentration of cadmium, evidencing the induction of acquired tolerance. The present study demonstrated the inducibility of B. glabrata HSP70 by cadmium, a relevant environmental contaminant, at non-lethal levels, providing evidences that the assessment of HSP70 in B. glabrata can be regarded as a suitable biomarker for ecotoxicological studies.

Keywords: Acquired resistance; Bioindicator; Biomarker; Mollusk; Protein induction.

MeSH terms

Animals
Biomphalaria / drug effects*
Biomphalaria / metabolism
Cadmium / metabolism
Cadmium / toxicity*
Dose-Response Relationship, Drug
Drug Tolerance
Ecotoxicology
Environmental Monitoring / methods*
Fresh Water / chemistry
HSP70 Heat-Shock Proteins / biosynthesis*
HSP70 Heat-Shock Proteins / metabolism
Heat-Shock Response*
Hot Temperature
Oxidative Stress / drug effects
Reproduction / drug effects
Water Pollutants, Chemical / metabolism
Water Pollutants, Chemical / toxicity*

Substances

HSP70 Heat-Shock Proteins
Water Pollutants, Chemical
Cadmium