Fatty acid synthase inhibits the O- GlcNAcase during oxidative stress

J Biol Chem. 2017 Apr 21;292(16):6493-6511. doi: 10.1074/jbc.M116.760785. Epub 2017 Feb 23.


The dynamic post-translational modification O-linked β-N-acetylglucosamine (O-GlcNAc) regulates thousands of nuclear, cytoplasmic, and mitochondrial proteins. Cellular stress, including oxidative stress, results in increased O-GlcNAcylation of numerous proteins, and this increase is thought to promote cell survival. The mechanisms by which the O-GlcNAc transferase (OGT) and the O-GlcNAcase (OGA), the enzymes that add and remove O-GlcNAc, respectively, are regulated during oxidative stress to alter O-GlcNAcylation are not fully characterized. Here, we demonstrate that oxidative stress leads to elevated O-GlcNAc levels in U2OS cells but has little impact on the activity of OGT. In contrast, the expression and activity of OGA are enhanced. We hypothesized that this seeming paradox could be explained by proteins that bind to and control the local activity or substrate targeting of OGA, thereby resulting in the observed stress-induced elevations of O-GlcNAc. To identify potential protein partners, we utilized BioID proximity biotinylation in combination with stable isotopic labeling of amino acids in cell culture (SILAC). This analysis revealed 90 OGA-interacting partners, many of which exhibited increased binding to OGA upon stress. The associations of OGA with fatty acid synthase (FAS), filamin-A, heat shock cognate 70-kDa protein, and OGT were confirmed by co-immunoprecipitation. The pool of OGA bound to FAS demonstrated a substantial (∼85%) reduction in specific activity, suggesting that FAS inhibits OGA. Consistent with this observation, FAS overexpression augmented stress-induced O-GlcNAcylation. Although the mechanism by which FAS sequesters OGA remains unknown, these data suggest that FAS fine-tunes the cell's response to stress and injury by remodeling cellular O-GlcNAcylation.

Keywords: BioID; O-GlcNAcylation; fatty acid synthase (FAS); glycoprotein; mgea5; oxidative stress; post-translational modification (PTM); protein-protein interaction; proteomics; signal transduction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biotinylation
  • Catalysis
  • Catalytic Domain
  • Cell Line, Tumor
  • Fatty Acid Synthases / metabolism*
  • Filamins / metabolism
  • Fluorescent Antibody Technique, Indirect
  • Glycoproteins / metabolism
  • HSC70 Heat-Shock Proteins / metabolism
  • Humans
  • Isoenzymes / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • N-Acetylglucosaminyltransferases / metabolism*
  • Oxidative Stress*
  • Proteomics
  • Signal Transduction
  • Tandem Mass Spectrometry


  • FLNA protein, human
  • Filamins
  • FlnA protein, mouse
  • Glycoproteins
  • HSC70 Heat-Shock Proteins
  • HSPA8 protein, human
  • Hspa8 protein, mouse
  • Isoenzymes
  • Fatty Acid Synthases
  • N-Acetylglucosaminyltransferases
  • O-GlcNAc transferase