Genetic and phenotypic characterization of mutants in four essential genes that map to the left half of HSV-1 UL DNA

Virology. 1987 Nov;161(1):198-210. doi: 10.1016/0042-6822(87)90186-3.

Abstract

Several HSV-1 proteins including the major capsid protein (VP5), two minor capsid proteins (VP11-12 and VP18.8), the alkaline nuclease and glycoprotein gH have been reported to be encoded by the left-most one-third of HSV-1 UL DNA. In this paper, we present physical mapping data and phenotypic analysis of six ts mutants whose mutations lie within this region and which collectively represent four functional complementation groups (1-6, 1-7, 1-10, and 1-26). In this study, mutants in complementation group 1-10 were found to be defective in the synthesis of viral DNA, late viral polypeptides, and the formation of mature capsid-like structures--properties characteristic of other ts mutants defective in functions required for viral DNA synthesis. Two DNA-positive mutants in complementation group 1-7 fail to induce capsid formation and probably possess mutations in coding sequences for VP5. Mutants in two other complementation groups (1-6 and 1-26) synthesize significant levels of viral DNA, late polypeptides, and capsids. The functions of the gene products represented by these mutants remain to be determined.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Capsid / biosynthesis
  • Capsid / genetics
  • Cell Line
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • DNA, Viral / biosynthesis
  • DNA, Viral / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Genes, Viral*
  • Genetic Complementation Test
  • Humans
  • Microscopy, Electron
  • Mutation
  • Phenotype
  • Plasmids
  • Simplexvirus / genetics*
  • Simplexvirus / growth & development
  • Simplexvirus / ultrastructure
  • Vero Cells
  • Viral Proteins / analysis

Substances

  • DNA, Viral
  • Viral Proteins
  • DNA Restriction Enzymes