Suppressor of rid1 (SID1) shares common targets with RID1 on florigen genes to initiate floral transition in rice

PLoS Genet. 2017 Feb 24;13(2):e1006642. doi: 10.1371/journal.pgen.1006642. eCollection 2017 Feb.

Abstract

The transition from vegetative to reproductive growth is a critical process in the life cycle of higher plants. Previously, we cloned Rice Indeterminate 1 (RID1), which acts as the master switch for the transition from the vegetative to reproductive phase in rice. Although the photoperiod pathway of RID1 inducing expression of the florigen genes Hd3a and RFT1 via Ehd1 has been established, the alternative pathways for the essential flowering transition need to be further examined. Here, we identified a Suppressor of rid1 (SID1), which rescues the never-flowering phenotype of rid1. SID1 encodes an INDETERMINATE DOMAIN (IDD) transcription factor. Mutation in SID1 showed the delayed flowering phenotype. Gain-of-function of SID1, OsIDD1, or OsIDD6 could restore the rid1 to flowering. Further analyses showed SID1 and RID1 directly target the promoter regions of Hd3a and RFT1, two florigen genes in rice. Taken together, our results reveal an autonomous flowering pathway might be mediated by RID1, thereby controlling the phase transition from vegetative to reproductive development in rice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Florigen / metabolism*
  • Flowers / genetics*
  • Flowers / metabolism
  • Flowers / radiation effects
  • Gene Expression Regulation, Plant*
  • Mutation
  • Oryza / genetics*
  • Oryza / metabolism
  • Oryza / radiation effects
  • Phenotype
  • Photoperiod
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / genetics
  • Signal Transduction / radiation effects
  • Time Factors
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism

Substances

  • Florigen
  • Plant Proteins
  • Transcription Factors

Grants and funding

This work was funded by the National Key Research and Development Program of China (2016YFD0100903), National Natural Science Foundation of China (31370222, 31425018), National Program on the Development of Basic Research (2013CB126900), Specialized Research Fund for the Doctoral Program of Higher Education (20130146110012) and Fundamental Research Funds for the Central Universities (2015PY003, 2013PY062). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.