The role of vitamin D3 in the proliferation of a human colon cancer cell line in vitro

Anticancer Res. Jul-Aug 1987;7(4B):817-21.

Abstract

LoVo, a cultured colon cancer cell line, is shown to possess a receptor for 1,25-dihydroxy vitamin D3 (1 alpha,25(OH)2D3) with a low capacity (28 fmol/mg protein) and high affinity (Kd: 1.9 x 10(-21)0M). When these cells were grown in monolayer culture in a chemically defined serum-free medium, a significant inhibition of proliferation was seen in the presence of 10 nM to 1 microM of 1 alpha,25(OH)2D3 (p less than 0.005. Furthermore, 1 alpha,25(OH)2D3 delayed early attachment of cells. After 8 days of treatment, aggregated cuboidal cells showed a marked change to an apparently spindle like morphology. The 1 alpha,25(OH)2D3 growth-inhibitory effect was modulated by verapamil (1 microM), a calcium channel blocker, hydrocortisone (1 microM), and moxestrol (1 mM), an estrogen analogue, and 2% charcoal-treated fetal bovine serum. This study represents the first demonstration of 1 alpha,25(OH)2D3 modulation of growth of human colon cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Calcitriol / pharmacology*
  • Carcinoma / pathology*
  • Cell Adhesion / drug effects
  • Cell Division / drug effects
  • Colonic Neoplasms / pathology*
  • Ethinyl Estradiol / analogs & derivatives
  • Ethinyl Estradiol / pharmacology
  • Growth Inhibitors*
  • Humans
  • Hydrocortisone / pharmacology
  • Receptors, Calcitriol
  • Receptors, Steroid / metabolism
  • Tumor Cells, Cultured
  • Verapamil / pharmacology

Substances

  • Growth Inhibitors
  • Receptors, Calcitriol
  • Receptors, Steroid
  • Ethinyl Estradiol
  • moxestrol
  • Verapamil
  • Calcitriol
  • Hydrocortisone