1 H, 13 C and 15 N backbone chemical shift assignments of camelid single-domain antibodies against active state µ-opioid receptor

Biomol NMR Assign. 2017 Apr;11(1):117-121. doi: 10.1007/s12104-017-9733-z. Epub 2017 Feb 26.

Abstract

Nanobodies are single chain antibodies that have become a highly valuable and versatile tool for biomolecular and therapeutic research. One application field is the stabilization of active states of flexible proteins, among which G-protein coupled receptors represent a very important class of membrane proteins. Here we present the backbone and side-chain assignment of the 1H, 13C and 15N resonances of Nb33 and Nb39, two nanobodies that recognize and stabilize the µ-opioid receptor to opioids in its active agonist-bound conformation. In addition, we present a comparison of their secondary structures as derived from NMR chemical shifts.

Keywords: Camelid antibody; G protein coupled receptor; Nanobody.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Camelidae*
  • Nuclear Magnetic Resonance, Biomolecular*
  • Receptors, Opioid, mu / immunology*
  • Single-Domain Antibodies / chemistry*
  • Single-Domain Antibodies / immunology*

Substances

  • Receptors, Opioid, mu
  • Single-Domain Antibodies