Lycium barbarum L. Polysaccharide (LBP) Reduces Glucose Uptake via Down-Regulation of SGLT-1 in Caco2 Cell

Molecules. 2017 Feb 22;22(2):341. doi: 10.3390/molecules22020341.

Abstract

Lycium barbarum L. polysaccharide (LBP) is prepared from Lycium barbarum L. (L. barbarum), which is a traditional Chinese medicine. LPB has been shown to have hypoglycemic effects. In order to gain some mechanistic insights on the hypoglycemic effects of LBP, we investigated the uptake of LBP and its effect on glucose absorption in the human intestinal epithelial cell line Caco2 cell. The uptake of LBP through Caco2 cell monolayer was time-dependent and was inhibited by phloridzin, a competitive inhibitor of SGLT-1. LPB decreased the absorption of glucose in Caco2 cell, and down-regulated the expression of SGLT-1. These results suggest that LBP might be transported across the human intestinal epithelium through SGLT-1 and it inhibits glucose uptake via down-regulating SGLT-1.

Keywords: Caco2 cell monolayer; GLUT-2; Lycium barbarum L. polysaccharide; SGLT-1.

MeSH terms

  • Caco-2 Cells
  • Carbohydrate Metabolism
  • Down-Regulation
  • Drugs, Chinese Herbal / pharmacology*
  • Glucose / metabolism*
  • Glucose Transporter Type 2 / metabolism
  • Humans
  • Hypoglycemic Agents / pharmacology*
  • Intestinal Absorption
  • Lycium / chemistry*
  • RNA, Messenger / genetics
  • Sodium-Glucose Transporter 1 / metabolism

Substances

  • Drugs, Chinese Herbal
  • Glucose Transporter Type 2
  • Hypoglycemic Agents
  • RNA, Messenger
  • SLC2A2 protein, human
  • SLC5A1 protein, human
  • Sodium-Glucose Transporter 1
  • lycium barbarum polysaccharide
  • Glucose