Objective: To study the relationship between XRCC1 gene polymorphism and DNA damage in peripheral blood lymphocytes of workers exposed to coal tar pitch. Methods: 203 coal tar asphalt device operation area workers (exposure group) and 76 logistics management personnel (control group) as the research ob-ject, determination of 1-hydroxypyrene concentrations in the urine as polycyclic aromatic hydrocarbons expo-sure dose, using the alkaline comet assay evaluation a peripheral blood lymphocyte DNA damage degree, using TaqMan MGB real time PCR method to detect XRCC1 gene 3 loci (XRCC1-194, XRCC1-280 and XRCC1-399) single nucleotide polymorphism. Results: No significant differences was observed in age, sex, smoking and alco-hol consumption between the two groups (P>0.05). The level of 1-OHP in the exposed group was significantly higher than that in the control group (1.27±0.93 μg/g creatinine) (P<0.05). The comet Olive tail moment level (3.21±0.93) in the peripheral blood lymphocytes of the exposed group was significantly higher than that in the control group (0.94 ± 0.39) (P<0.05). There was no significant difference in genotype distribution of XRCC1-194, XRCC1-280 and XRCC1-399 between the two groups (P>0.05). There was a significant correlation be-tween the XRCC1-280 locus gene polymorphism and comet Olive tail moment in the exposure group (P<0.05) af-ter adjustment for sex, age, smoking rate, drinking rate, length of service and urinary 1-OHP concentration. The comet Olive tail moment level of GG individuals carrying wild homozygous genotype was significantly lower than that of individuals carrying heterozygous genotype GA and carrying mutant homozygous genotype AA (P< 0.05) , and the difference was statistically significant (P<0.05) The comet Olive tail moment level of heterozy-gous genotype GA was significantly lower than that of genotype AA with mutational homozygous genotype AA (P<0.05) , and the difference was statistically significant (P<0.05). Conclusion: Arg280His locus polymor-phism of XRCC1 gene may influence the DNA damage level of peripheral blood lymphocytes induced by occupational exposure to coal tar pitch.
目的：研究煤焦油沥青接触工人XRCC1基因多态性与外周血淋巴细胞DNA损伤易感性的关系。 方法：选择203名煤焦油沥青装置区作业工人（接触组）和76名后勤管理人员（对照组）为研究对象。使用统一调查表收集个人基本信息。用碱水解-高效液相色谱法（HPLC）测定尿中1-羟基芘（1-OHP）浓度作为多环芳烃接触内剂量，用碱性彗星实验评价个体外周血淋巴细胞DNA损伤程度，用荧光探针实时定量PCR实验方法（TaqMan MGB real-time PCR）检测XRCC1基因3个位点（XRCC1-194、XRCC1-280、XRCC1-399）的单核苷酸多态性。 结果：两组研究对象年龄、性别构成、吸烟率及饮酒率比较，差异均无统计学意义（P>0.05 ）。接触组尿中1-OHP浓度（2.47±0.93μg/g肌酐）明显高于对照组（1.23±0.74μg/g肌酐），差异有统计学意义（P<0.05）。接触组外周血淋巴细胞彗星Olive尾矩水平（3.21±0.93）明显高于对照组（0.94±0.39），差异有统计学意义（P<0.05）。两组研究对象XRCC1-194、XRCC1-280、XRCC1-399等3个位点各基因型在两组中的分布差异均无统计学意义（P>0.05）。在校正性别、年龄、吸烟率、饮酒率、工龄和尿中1-OHP浓度后，接触组中XRCC1-280位点基因多态性和彗星Olive尾矩水平有明显关联性（P< 0.05）。经两两比较，携带野生纯合子基因型GG个体彗星Olive尾矩水平明显低于携带杂合子基因型GA及携带突变纯合子基因型AA的个体，差异有统计学意义（P<0.05）；携带杂合子基因型GA个体彗星Olive尾矩水平明显低于携带突变纯合子基因型AA的个体，差异有统计学意义（P<0.05）。 结论： XRCC1基因Arg280His位点多态性可影响煤焦油沥青职业暴露导致的外周血淋巴细胞DNA损伤水平。.
Keywords: Coal tar; DNA damage; Pitch; Polymorphisms, single nucle otide.