Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

doi:10.1007/978-1-4939-6820-6_8

. 2017:1566:77-85.

doi: 10.1007/978-1-4939-6820-6_8.

Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

Farnaz Shamsi¹, Yu-Hua Tseng²

Affiliations

¹ Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA, 02215, USA.
² Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. yu-hua.tseng@joslin.harvard.edu.

PMID: 28244042
PMCID: PMC5870121
DOI: 10.1007/978-1-4939-6820-6_8

Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

Farnaz Shamsi et al. Methods Mol Biol. 2017.

. 2017:1566:77-85.

doi: 10.1007/978-1-4939-6820-6_8.

Authors

Farnaz Shamsi¹, Yu-Hua Tseng²

Affiliations

¹ Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA, 02215, USA.
² Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. yu-hua.tseng@joslin.harvard.edu.

PMID: 28244042
PMCID: PMC5870121
DOI: 10.1007/978-1-4939-6820-6_8

Abstract

Human brown and white preadipocytes offer unique cell models to study human adipogenesis and thermogenesis. Here, we describe the detailed procedures for isolation of human brown and white predipocytes from deep and superficial neck fat. To grow these cells in vitro for a prolonged period of time, they should be immortalized following the procedure discussed here. We also provide the protocol for expansion, cryopreservation, and adipogenic differentiation of cells.

Keywords: Brown and white adipose tissue; Differentiation; Immortalization; Neck fat; Preadipocytes; Stromal vascular fraction (SVF).

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References

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1. Nedergaard J, Bengtsson T, Cannon B. Unexpected evidence for active brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab. 2007;293(2):E444–452. doi: 10.1152/ajpendo.00691.2006. - DOI - PubMed
1. Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB, Kuo FC, Palmer EL, Tseng YH, Doria A, Kolodny GM, Kahn CR. Identification and importance of brown adipose tissue in adult humans. N Engl J Med. 2009;360(15):1509–1517. doi: 10.1056/NEJMoa0810780. - DOI - PMC - PubMed
1. van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, Drossaerts JM, Kemerink GJ, Bouvy ND, Schrauwen P, Teule GJ. Cold-activated brown adipose tissue in healthy men. N Engl J Med. 2009;360(15):1500–1508. doi: 10.1056/NEJMoa0808718. - DOI - PubMed
1. Virtanen KA, Lidell ME, Orava J, Heglind M, Westergren R, Niemi T, Taittonen M, Laine J, Savisto NJ, Enerback S, Nuutila P. Functional brown adipose tissue in healthy adults. N Engl J Med. 2009;360(15):1518–1525. doi: 10.1056/NEJMoa0808949. - DOI - PubMed

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[1] Orava J, Nuutila P, Lidell ME, Oikonen V, Noponen T, Viljanen T, Scheinin M, Taittonen M, Niemi T, Enerback S, Virtanen KA. Different metabolic responses of human brown adipose tissue to activation by cold and insulin. Cell Metab. 2011;14(2):272–279. doi: 10.1016/j.cmet.2011.06.012. - DOI - PubMed

[2] Orava J, Nuutila P, Lidell ME, Oikonen V, Noponen T, Viljanen T, Scheinin M, Taittonen M, Niemi T, Enerback S, Virtanen KA. Different metabolic responses of human brown adipose tissue to activation by cold and insulin. Cell Metab. 2011;14(2):272–279. doi: 10.1016/j.cmet.2011.06.012. - DOI - PubMed

[3] Nedergaard J, Bengtsson T, Cannon B. Unexpected evidence for active brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab. 2007;293(2):E444–452. doi: 10.1152/ajpendo.00691.2006. - DOI - PubMed

[4] Nedergaard J, Bengtsson T, Cannon B. Unexpected evidence for active brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab. 2007;293(2):E444–452. doi: 10.1152/ajpendo.00691.2006. - DOI - PubMed

[5] Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB, Kuo FC, Palmer EL, Tseng YH, Doria A, Kolodny GM, Kahn CR. Identification and importance of brown adipose tissue in adult humans. N Engl J Med. 2009;360(15):1509–1517. doi: 10.1056/NEJMoa0810780. - DOI - PMC - PubMed

[6] Cypess AM, Lehman S, Williams G, Tal I, Rodman D, Goldfine AB, Kuo FC, Palmer EL, Tseng YH, Doria A, Kolodny GM, Kahn CR. Identification and importance of brown adipose tissue in adult humans. N Engl J Med. 2009;360(15):1509–1517. doi: 10.1056/NEJMoa0810780. - DOI - PMC - PubMed

[7] van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, Drossaerts JM, Kemerink GJ, Bouvy ND, Schrauwen P, Teule GJ. Cold-activated brown adipose tissue in healthy men. N Engl J Med. 2009;360(15):1500–1508. doi: 10.1056/NEJMoa0808718. - DOI - PubMed

[8] van Marken Lichtenbelt WD, Vanhommerig JW, Smulders NM, Drossaerts JM, Kemerink GJ, Bouvy ND, Schrauwen P, Teule GJ. Cold-activated brown adipose tissue in healthy men. N Engl J Med. 2009;360(15):1500–1508. doi: 10.1056/NEJMoa0808718. - DOI - PubMed

[9] Virtanen KA, Lidell ME, Orava J, Heglind M, Westergren R, Niemi T, Taittonen M, Laine J, Savisto NJ, Enerback S, Nuutila P. Functional brown adipose tissue in healthy adults. N Engl J Med. 2009;360(15):1518–1525. doi: 10.1056/NEJMoa0808949. - DOI - PubMed

[10] Virtanen KA, Lidell ME, Orava J, Heglind M, Westergren R, Niemi T, Taittonen M, Laine J, Savisto NJ, Enerback S, Nuutila P. Functional brown adipose tissue in healthy adults. N Engl J Med. 2009;360(15):1518–1525. doi: 10.1056/NEJMoa0808949. - DOI - PubMed

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Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

Affiliations

Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

Authors

Affiliations

Abstract

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