Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines

Methods Mol Biol. 2017;1566:77-85. doi: 10.1007/978-1-4939-6820-6_8.


Human brown and white preadipocytes offer unique cell models to study human adipogenesis and thermogenesis. Here, we describe the detailed procedures for isolation of human brown and white predipocytes from deep and superficial neck fat. To grow these cells in vitro for a prolonged period of time, they should be immortalized following the procedure discussed here. We also provide the protocol for expansion, cryopreservation, and adipogenic differentiation of cells.

Keywords: Brown and white adipose tissue; Differentiation; Immortalization; Neck fat; Preadipocytes; Stromal vascular fraction (SVF).

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adipocytes, Brown / cytology*
  • Adipocytes, White / cytology*
  • Cell Culture Techniques
  • Cell Differentiation*
  • Cell Line, Transformed
  • Gene Expression
  • Humans
  • Stem Cells / cytology*
  • Stem Cells / metabolism*
  • Telomerase / genetics
  • Transfection


  • Telomerase