Oral Ingestion of Collagen Hydrolysate Leads to the Transportation of Highly Concentrated Gly-Pro-Hyp and Its Hydrolyzed Form of Pro-Hyp into the Bloodstream and Skin

J Agric Food Chem. 2017 Mar 22;65(11):2315-2322. doi: 10.1021/acs.jafc.6b05679. Epub 2017 Mar 8.


Collagen hydrolysate is a well-known dietary supplement for the treatment of skin aging; however, its mode of action remains unknown. Previous studies have shown that the oral ingestion of collagen hydrolysate leads to elevated levels of collagen-derived peptides in the blood, but whether these peptides reach the skin remains unclear. Here, we analyzed the plasma concentration of collagen-derived peptides after ingestion of high tripeptide containing collagen hydrolysate in humans. We identified 17 types of collagen-derived peptides transiently, with a particular enrichment in Gly-Pro-Hyp. This was also observed using an in vivo mouse model in the plasma and skin, albeit with a higher enrichment of Pro-Hyp in the skin. Interestingly, this Pro-Hyp enrichment in the skin was derived from Gly-Pro-Hyp hydrolysis, as the administration of pure Gly-Pro-Hyp peptide led to similar results. Therefore, we propose that functional peptides can be transferred to the skin by dietary supplements of collagen.

Keywords: Gly-Pro-Hyp; Pro-Hyp; collagen; dietary supplement; kinetics; peptide.

MeSH terms

  • Adult
  • Animals
  • Collagen / chemistry*
  • Collagen / metabolism*
  • Dipeptides / blood
  • Dipeptides / metabolism*
  • Eating
  • Female
  • Humans
  • Hydrolysis
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Oligopeptides / blood
  • Oligopeptides / metabolism*
  • Protein Hydrolysates / chemistry
  • Protein Hydrolysates / metabolism*
  • Skin / metabolism*
  • Young Adult


  • Dipeptides
  • Oligopeptides
  • Protein Hydrolysates
  • glycyl-prolyl-hydroxyproline
  • prolyl-4-hydroxyproline
  • Collagen