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, 35 (5), 438-440

Precise Base Editing in Rice, Wheat and Maize With a Cas9-cytidine Deaminase Fusion

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Precise Base Editing in Rice, Wheat and Maize With a Cas9-cytidine Deaminase Fusion

Yuan Zong et al. Nat Biotechnol.

Abstract

Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.

Comment in

  • Base editing on the rise.
    May A. May A. Nat Biotechnol. 2017 May 9;35(5):428-429. doi: 10.1038/nbt.3871. Nat Biotechnol. 2017. PMID: 28486457 No abstract available.

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