Fission yeast APC/C activators Slp1 and Fzr1 sequentially trigger two consecutive nuclear divisions during meiosis

FEBS Lett. 2017 Apr;591(7):1029-1040. doi: 10.1002/1873-3468.12612. Epub 2017 Mar 17.


In meiosis, two rounds of nuclear division occur consecutively without DNA replication between the divisions. We isolated a fission yeast mutant in which the nucleus divides only once to generate two spores, as opposed to four, in meiosis. In this mutant, we found that the initiation codon of the slp1+ gene is converted to ATA, producing a reduced amount of Slp1. As a member of the Fizzy family of anaphase-promoting complex/cyclosome (APC/C) activators, Slp1 is essential for vegetative growth; however, the mutant allele shows a phenotype only in meiosis. Slp1 insufficiency delays degradation of maturation-promoting factor at the first meiotic division, and another APC/C activator, Fzr1, which acts late in meiosis, terminates meiosis immediately after the delayed first division to produce two viable spores.

Keywords: Schizosaccharomyces pombe; APC/C; Fzr1; Mes1; Slp1; meiosis.

Publication types

  • Letter

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome / metabolism
  • Blotting, Western
  • Cdc20 Proteins / genetics
  • Cdc20 Proteins / metabolism*
  • Cdh1 Proteins / genetics
  • Cdh1 Proteins / metabolism*
  • Cell Nucleus Division / genetics
  • Meiosis*
  • Microscopy, Fluorescence
  • Mutation
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins / genetics
  • Schizosaccharomyces pombe Proteins / metabolism*
  • Spores, Fungal / genetics
  • Spores, Fungal / metabolism
  • Time Factors


  • Cdc20 Proteins
  • Cdh1 Proteins
  • Schizosaccharomyces pombe Proteins
  • slp1 protein, S pombe
  • Anaphase-Promoting Complex-Cyclosome