Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens

J Exp Med. 1987 Nov 1;166(5):1548-66. doi: 10.1084/jem.166.5.1548.

Abstract

Leukocyte common antigens (LCAs, also known as T200 and CD 45) are integral membrane proteins expressed exclusively on hematopoietic cells. These molecules exhibit varying molecular masses and epitopes when expressed in different cell types. To determine the genetic bases for the generation of this diversity, three classes of human LCA cDNA clones that are different near their 5' ends have been isolated. These differences arose as a result of differential usage of three exons as determined from an analysis of a genomic DNA clone. Furthermore, Northern blot analysis with LCA exon-specific probes demonstrates the existence of at least two more LCA mRNA forms that are generated by differential splicing. A comparison of the human and mouse LCA protein sequences revealed a marked difference only in the extracellular domain.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / immunology
  • Bacteriophage lambda / genetics
  • Base Sequence
  • Cell Line
  • DNA / genetics
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Exons*
  • Histocompatibility Antigens / genetics*
  • Histocompatibility Antigens / immunology
  • Humans
  • Leukocyte Common Antigens
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics*
  • Sequence Homology, Nucleic Acid
  • T-Lymphocytes / immunology

Substances

  • DNA, Recombinant
  • Histocompatibility Antigens
  • RNA, Messenger
  • DNA
  • DNA Restriction Enzymes
  • Leukocyte Common Antigens

Associated data

  • GENBANK/Y00638