Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors

Methods Mol Biol. 2017;1556:103-116. doi: 10.1007/978-1-4939-6771-1_5.

Abstract

Uncovering how muscle stem cells behave in quiescent and activated states is central to understand the basic rules governing normal muscle development and regeneration in pathological conditions. Specification of mesodermal lineages including muscle stemlike adult muscle precursors (AMPs) has been extensively studied in Drosophila providing an attractive framework for investigating muscle stem cell properties. Restricted number of AMP cells, relative ease in following their behavior, and large number of genetic tools available make fruit fly an attractive model system for studying muscle stem cells. In this chapter, we describe the recently developed tools to visualize and target the body wall and imaginal AMPs.

Keywords: Adult muscle precursors (AMPs); Drosophila; Imaging; Muscle development; Muscle stem cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism
  • Animals
  • Biomarkers
  • Drosophila / cytology*
  • Fluorescent Antibody Technique
  • Microscopy, Fluorescence
  • Molecular Imaging / methods
  • Muscle Development
  • Muscles / cytology*
  • Myoblasts / cytology
  • Myoblasts / metabolism
  • Phenotype
  • Stem Cells / cytology*
  • Stem Cells / metabolism*

Substances

  • Biomarkers