Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture

Methods Mol Biol. 2017;1556:179-189. doi: 10.1007/978-1-4939-6771-1_9.

Abstract

Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence -activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.

Keywords: Cell culture; FACS; Fibro/adipogenic progenitors; Mesenchymal stromal cell; Skeletal muscle.

MeSH terms

  • Adipogenesis
  • Adult Stem Cells / cytology
  • Adult Stem Cells / metabolism
  • Animals
  • Biomarkers
  • Cell Culture Techniques
  • Cell Differentiation*
  • Cell Lineage
  • Cell Separation / methods
  • Flow Cytometry / methods
  • Immunophenotyping
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism
  • Mice
  • Muscle, Skeletal / cytology*
  • Stem Cells / cytology*
  • Stem Cells / metabolism*
  • Workflow

Substances

  • Biomarkers