A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia

Nature. 1987 Nov 26-Dec 2;330(6146):386-8. doi: 10.1038/330386a0.

Abstract

The leukaemic cells of more than 90% of chronic myelogenous leukaemia (CML) patients and of 10% of acute lymphocytic leukaemia (ALL) patients carry the t(9:22) (q34:q11) translocation which generates the Philadelphia chromosome (Ph1). In CML the abl gene is translocated from chromosome 9 to the centre of the bcr gene on chromosome 22 and this results in production of chimaeric bcr-abl RNA translated into a protein of relative molecular mass (Mr) 210,000 (210K). Our data indicate that in ALL abl is translocated into the 5' region of the bcr gene. The consequence of this is the expression of a fused transcript in which the first exon of bcr is linked to the second abl exon. This transcript encodes a 190K protein kinase.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chimera
  • DNA Restriction Enzymes
  • Genes*
  • Humans
  • Leukemia, Lymphoid / genetics*
  • Molecular Sequence Data
  • Philadelphia Chromosome*
  • Protein Biosynthesis
  • Protein Kinases / genetics*
  • RNA, Messenger / genetics
  • Transcription, Genetic*

Substances

  • RNA, Messenger
  • Protein Kinases
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X06418