MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

doi:10.1038/nmeth.4193

. 2017 Apr;14(4):331-332.

doi: 10.1038/nmeth.4193. Epub 2017 Feb 27.

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Shawn Q Zheng^{1

2}, Eugene Palovcak¹, Jean-Paul Armache¹, Kliment A Verba¹, Yifan Cheng^{1

2}, David A Agard^{1

2}

Affiliations

¹ Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
² Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.

PMID: 28250466
PMCID: PMC5494038
DOI: 10.1038/nmeth.4193

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Shawn Q Zheng et al. Nat Methods. 2017 Apr.

. 2017 Apr;14(4):331-332.

doi: 10.1038/nmeth.4193. Epub 2017 Feb 27.

Authors

Shawn Q Zheng^{1

2}, Eugene Palovcak¹, Jean-Paul Armache¹, Kliment A Verba¹, Yifan Cheng^{1

2}, David A Agard^{1

2}

Affiliations

¹ Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
² Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA.

PMID: 28250466
PMCID: PMC5494038
DOI: 10.1038/nmeth.4193

No abstract available

PubMed Disclaimer

Conflict of interest statement

Competing Financial Interests:

The autors decalre no competing financial interest.

Figures

**Figure 1. Local motion correction by MotionCor2**
(a) Schematic drawing illustrates that, when the sample is tilted, the observable motions in the image plan is the projection of z-motion produced by doming of the sample under electron beam. (b) Image of frozen hydrated archaeal 20S proteasome overlaid with the traces of global motion based upon whole frame alignment (long trace originated from the center of image) and each patch predicted from the polynomial function. (c) Fourier Shell Correlation (FSC) curves of 3D reconstructions determined using micrographs corrected by Unblur with dose weighting, Unblur followed by particle polishing, correction by MotionCor2 with dose-weighting, MotionCor2 followed by particle polishing and MotionCor2 with per-frame B-factor weighting. The color code is marked in the figure. (d) 3D reconstruction of archaeal 20S proteasome filtered to 2.5Å resolution and sharpened by a temperature factor of –103.8Å². (e) Density of a β-sheet, where the main chain carbonyl is colored in red. Visualization of main chain carbonyl density requires better a resolution that is better than 3Å. (f) Representative side chain densities.

See this image and copyright information in PMC

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References

1. Cheng Y. Single-Particle Cryo-EM at Crystallographic Resolution. Cell. 2015;161:450–457. doi: 10.1016/j.cell.2015.03.049. - DOI - PMC - PubMed
1. Kuhlbrandt W. Biochemistry. The resolution revolution. Science. 2014;343:1443–1444. doi: 10.1126/science.1251652. - DOI - PubMed
1. Brilot AF, et al. Beam-induced motion of vitrified specimen on holey carbon film. J Struct Biol. 2012;177:630–637. doi: 10.1016/j.jsb.2012.02.003. - DOI - PMC - PubMed
1. Li X, et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nature Methods. 2013;10:584–590. - PMC - PubMed
1. Liao M, Cao E, Julius D, Cheng Y. Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature. 2013;504:107–112. doi: 10.1038/nature12822. - DOI - PMC - PubMed

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[1] Cheng Y. Single-Particle Cryo-EM at Crystallographic Resolution. Cell. 2015;161:450–457. doi: 10.1016/j.cell.2015.03.049. - DOI - PMC - PubMed

[2] Cheng Y. Single-Particle Cryo-EM at Crystallographic Resolution. Cell. 2015;161:450–457. doi: 10.1016/j.cell.2015.03.049. - DOI - PMC - PubMed

[3] Kuhlbrandt W. Biochemistry. The resolution revolution. Science. 2014;343:1443–1444. doi: 10.1126/science.1251652. - DOI - PubMed

[4] Kuhlbrandt W. Biochemistry. The resolution revolution. Science. 2014;343:1443–1444. doi: 10.1126/science.1251652. - DOI - PubMed

[5] Brilot AF, et al. Beam-induced motion of vitrified specimen on holey carbon film. J Struct Biol. 2012;177:630–637. doi: 10.1016/j.jsb.2012.02.003. - DOI - PMC - PubMed

[6] Brilot AF, et al. Beam-induced motion of vitrified specimen on holey carbon film. J Struct Biol. 2012;177:630–637. doi: 10.1016/j.jsb.2012.02.003. - DOI - PMC - PubMed

[7] Li X, et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nature Methods. 2013;10:584–590. - PMC - PubMed

[8] Li X, et al. Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nature Methods. 2013;10:584–590. - PMC - PubMed

[9] Liao M, Cao E, Julius D, Cheng Y. Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature. 2013;504:107–112. doi: 10.1038/nature12822. - DOI - PMC - PubMed

[10] Liao M, Cao E, Julius D, Cheng Y. Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature. 2013;504:107–112. doi: 10.1038/nature12822. - DOI - PMC - PubMed

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MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Affiliations

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Authors

Affiliations

Conflict of interest statement

Figures

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