CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through modulation of TGF-β signaling

Sci Rep. 2017 Mar 3;7:43851. doi: 10.1038/srep43851.

Abstract

Previous studies have shown that CD61 (integrin-β3) promotes the differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) into germ-like cells. However, the mechanism remains unclear. In this study, we showed that overexpression of CD61 in canine adipose-derived mesenchymal stem cells (cADMSCs) promotes their differentiation into primordial germ cell (PGC)-like cells. Quantitative real-time PCR, immunocytochemistry and western blot detected higher levels of PGC-specific markers in CD61-overexpressed cADMSCs compared with those in control cells. Moreover, phosphorylation of Smad2, a downstream mediator of transforming growth factor beta (TGF-β), was increased in CD61-overexpressed cADMSCs than that in control cells. However, the expression of PGC-specific markers was downregulated in cADMSCs treated with a TGF-β inhibitor. These results suggested that CD61 could induce cADMSCs to differentiate into PGC-like cells by relying on the activation of TGF-β pathway. ADMSCs possess a considerable potential in treating the infertility of rare animal species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology
  • Animals
  • Cell Differentiation*
  • Cells, Cultured
  • Dogs
  • Gene Expression / drug effects
  • Germ Cells / cytology
  • Germ Cells / metabolism*
  • Integrin beta3 / genetics
  • Integrin beta3 / metabolism*
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Phosphorylation
  • Pyrazoles / pharmacology
  • Pyrroles / pharmacology
  • Signal Transduction / drug effects
  • Smad2 Protein / metabolism
  • Transforming Growth Factor beta / metabolism*
  • Transforming Growth Factor beta / pharmacology

Substances

  • Integrin beta3
  • LY2109761
  • Pyrazoles
  • Pyrroles
  • Smad2 Protein
  • Transforming Growth Factor beta