Involvement of GTP-binding "G" proteins in transport through the Golgi stack

Cell. 1987 Dec 24;51(6):1053-62. doi: 10.1016/0092-8674(87)90591-5.


GTP gamma S irreversibly inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. Fluoride, potentiated by the addition of aluminum ion, also causes a strong inhibition. These are hallmarks of the involvement of a guanine nucleotide-binding or regulatory "G" protein. Inhibition by GTP gamma S requires a cytosolic inhibitory factor that binds to Golgi membranes during inhibition. Preincubation experiments reveal that GTP gamma S blocks the function of acceptor Golgi but not donor Golgi membranes. More specifically, a processing step in between vesicle attachment and the actual fusion event seems to be affected. Electron microscopy demonstrates a corresponding 5-fold accumulation of non-clathrin-coated buds and vesicles associated with the Golgi cisternae during inhibition by GTP gamma S.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aluminum / pharmacology
  • Aluminum Compounds*
  • Animals
  • Biological Transport / drug effects
  • Cell Line
  • Cell-Free System
  • Fluorides / pharmacology
  • GTP-Binding Proteins / metabolism*
  • Golgi Apparatus / metabolism*
  • Golgi Apparatus / ultrastructure
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Guanosine Triphosphate / analogs & derivatives
  • Guanosine Triphosphate / pharmacology
  • Intracellular Membranes / metabolism
  • Membrane Glycoproteins*
  • Microscopy, Electron
  • Thionucleotides / pharmacology
  • Vesicular stomatitis Indiana virus
  • Viral Envelope Proteins*
  • Viral Matrix Proteins / metabolism*


  • Aluminum Compounds
  • G protein, vesicular stomatitis virus
  • Membrane Glycoproteins
  • Thionucleotides
  • Viral Envelope Proteins
  • Viral Matrix Proteins
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Guanosine Triphosphate
  • Aluminum
  • GTP-Binding Proteins
  • Fluorides
  • aluminum fluoride