Inhibition of dietary fat-promoted colon carcinogenesis in rats by supplemental calcium or vitamin D3

Carcinogenesis. 1988 Jan;9(1):187-90. doi: 10.1093/carcin/9.1.187.


A 2 X 2 X 2 factorial experimental design was used to investigate the effects of supplemental calcium (Ca) (0.5% versus 1.0% of diet as Ca gluconate) and vitamin D3 (D) (1000 IU/kg diet versus 2000 IU/kg diet) on 1,2-dimethylhydrazine-induced colon carcinogenesis in male F344 rats promoted with a 20% corn oil diet. Animals on the high-fat (HF) diet had an increased tumor incidence compared to the low-fat (LF) control diet (86% versus 53%, P less than 0.05) and supplemental Ca or D reduced this to or below the LF incidence (HF + Ca: 53%, HF + D: 47%). However, supplemental Ca or D had no inhibitory effect with LF diets (LF + Ca: 67%, LF + D: 60%). The results of this study indicate a possible role for supplemental Ca or D in the prevention of colon cancer, effective only in HF diets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / pharmacology*
  • Cholecalciferol / pharmacology*
  • Colonic Neoplasms / etiology*
  • Dietary Fats / adverse effects*
  • Drug Interactions
  • Male
  • Rats
  • Rats, Inbred F344


  • Dietary Fats
  • Cholecalciferol
  • Calcium