Bacterial flagella grow through an injection-diffusion mechanism

Elife. 2017 Mar 6;6:e23136. doi: 10.7554/eLife.23136.

Abstract

The bacterial flagellum is a self-assembling nanomachine. The external flagellar filament, several times longer than a bacterial cell body, is made of a few tens of thousands subunits of a single protein: flagellin. A fundamental problem concerns the molecular mechanism of how the flagellum grows outside the cell, where no discernible energy source is available. Here, we monitored the dynamic assembly of individual flagella using in situ labelling and real-time immunostaining of elongating flagellar filaments. We report that the rate of flagellum growth, initially ∼1,700 amino acids per second, decreases with length and that the previously proposed chain mechanism does not contribute to the filament elongation dynamics. Inhibition of the proton motive force-dependent export apparatus revealed a major contribution of substrate injection in driving filament elongation. The combination of experimental and mathematical evidence demonstrates that a simple, injection-diffusion mechanism controls bacterial flagella growth outside the cell.

Keywords: Salmonella enterica; bacterial flagellum; biophysics; continuous-flow immunostaining; infectious disease; injection-diffusion mechanism; microbiology; proton motive force; structural biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flagella / metabolism*
  • Flagellin / metabolism*
  • Models, Theoretical
  • Organelle Biogenesis*
  • Proton-Motive Force
  • Salmonella enterica / metabolism*

Substances

  • Flagellin