Expression of functional (Na+ + K+)-ATPase from cloned cDNAs

FEBS Lett. 1987 Dec 10;225(1-2):27-32. doi: 10.1016/0014-5793(87)81125-0.

Abstract

Functional (Na+ + K+)-ATPase is formed in Xenopus oocytes injected with alpha- and beta-subunit-specific mRNAs derived from cloned Torpedo californica cDNAs. Both the mRNAs are required for the expression of functional (Na+ + K+)-ATPase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / genetics*
  • DNA, Recombinant*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Gene Expression Regulation
  • Glycoside Hydrolases / metabolism
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
  • Microsomes / metabolism
  • Oocytes / metabolism*
  • Protein Biosynthesis
  • RNA, Messenger / genetics*
  • Recombination, Genetic
  • Sodium-Potassium-Exchanging ATPase / biosynthesis*
  • Sodium-Potassium-Exchanging ATPase / genetics
  • Torpedo / genetics*
  • Transcription, Genetic
  • Xenopus

Substances

  • DNA, Recombinant
  • RNA, Messenger
  • DNA
  • Glycoside Hydrolases
  • Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
  • Sodium-Potassium-Exchanging ATPase