Protective effects of aloperine on monocrotaline-induced pulmonary hypertension in rats

Biomed Pharmacother. 2017 May:89:632-641. doi: 10.1016/j.biopha.2017.02.033. Epub 2017 Mar 3.

Abstract

Pulmonary hypertension (PH) is serious, fatal disease which is promoted by oxidative stress. Aloperine have antioxidation effects, which effects on pulmonary arteries remain unclear. Therefore, this study is designed to investigate whether aloperine has protective effects on PH induced by monocrotaline and whether these effects are associated with oxidative stress. PH was induced by monocrotaline (60mg/kg), and subsequently oral administration of aloperine (25, 50, 100mg/kg/day). At the end of the experiment, hemodynamic, pathomorphologic, electrocardiographic and echocardiographic data from the rats were obtained. At same time, oxidative stress biomarkers (superoxide dismutase, malonyldialdehyde, catalase, glutathione peroxidase, total antioxidant capacity) and the protein expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX)-2, NOX-4 in the lung of rat has been detected. The result shows that aloperine treatment showed significantly improvement in hemodynamic, pathomorphologic, electrocardiographic and echocardiographic data. Moreover, aloperine treatment can alleviate the changes of oxidative stress biomarkers and suppress the expression levels of NOX-2, NOX-4. In summary, this study indicates that aloperine have protective effects on monocrotaline-induced PH. And these effects may be related to inhibit oxidative stress.

Keywords: Aloperine; Monocrotaline; NOX-2; NOX-4; Oxidative stress; Pulmonary hypertension.

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Biomarkers / analysis
  • Dose-Response Relationship, Drug
  • Echocardiography
  • Electrocardiography
  • Hypertension, Pulmonary / chemically induced*
  • Hypertension, Pulmonary / diagnostic imaging
  • Hypertension, Pulmonary / prevention & control*
  • Hypertrophy, Right Ventricular / diagnostic imaging
  • Hypertrophy, Right Ventricular / prevention & control
  • Male
  • Monocrotaline*
  • Oxidative Stress / drug effects
  • Piperidines / pharmacology*
  • Protective Agents / pharmacology*
  • Pulmonary Artery / metabolism
  • Pulmonary Artery / pathology
  • Pulmonary Circulation / drug effects
  • Quinolizidines
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Antioxidants
  • Biomarkers
  • Piperidines
  • Protective Agents
  • Quinolizidines
  • aloperine
  • Monocrotaline