GuideScan Software for Improved Single and Paired CRISPR Guide RNA Design

Nat Biotechnol. 2017 Apr;35(4):347-349. doi: 10.1038/nbt.3804. Epub 2017 Mar 6.

Abstract

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Algorithms*
  • CRISPR-Cas Systems / genetics
  • Chromosome Mapping / methods
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
  • Gene Silencing*
  • Machine Learning*
  • RNA, Small Interfering / genetics*
  • Sequence Analysis, RNA / methods
  • Software*

Substances

  • RNA, Small Interfering