AKIN10, a representative Arabidopsis SNF1-related protein kinase 1 (SnRK1), phosphorylates and downregulates plant HMG-CoA reductase

FEBS Lett. 2017 Apr;591(8):1159-1166. doi: 10.1002/1873-3468.12618. Epub 2017 Apr 3.

Abstract

HMG-CoA reductase (HMGR) is a key enzyme in the mevalonate pathway for sterols and cytosolic isoprenoid production. Although HMGR kinases from spinach, barley, and cauliflower tissues have been strongly suggested as members of SNF1-related protein kinases 1 (SnRK1), the phosphorylation and inactivation of HMGR by plant SnRK1s has not been demonstrated. In this study, we elucidated that AKIN10, an Arabidopsis SnRK1, acts as an HMGR kinase. The recombinant AKIN10 phosphorylates and inactivates AtHMGR1S using recombinant GRIK1 as the AKIN10 activator. In contrast, AKIN10-GRIK1 fails to inactivate AtHMGR1S-S577A, suggesting that this is achieved through Ser577 phosphorylation. Moreover, phosphorylation is detected not only in AtHMGR1S but also in AtHMGR1S-S577A, suggesting the presence of a novel regulatory mechanism of plant HMGR.

Keywords: HMGR; GRIK1; SnRK1; isoprenoid; phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Arabidopsis / enzymology*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / antagonists & inhibitors*
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Catalytic Domain
  • Conserved Sequence
  • Enzyme Activation
  • Enzyme Repression
  • Gene Expression Regulation, Plant*
  • Hevea / enzymology
  • Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / chemistry
  • Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / genetics
  • Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / metabolism
  • Mutation
  • Peptide Fragments / antagonists & inhibitors
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Phosphorylation
  • Plant Proteins / antagonists & inhibitors
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Protein Interaction Domains and Motifs
  • Protein Processing, Post-Translational*
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • Recombinant Proteins / metabolism
  • Serine / metabolism
  • Substrate Specificity

Substances

  • Arabidopsis Proteins
  • Peptide Fragments
  • Plant Proteins
  • Recombinant Proteins
  • Serine
  • HMGR protein, Arabidopsis
  • Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent
  • GRIK1 protein, Arabidopsis
  • Protein-Serine-Threonine Kinases
  • SnRK1 protein, Arabidopsis