MCR-1.6, a New MCR Variant Carried by an IncP Plasmid in a Colistin-Resistant Salmonella enterica Serovar Typhimurium Isolate from a Healthy Individual

Xin Lu; Yongfei Hu; Ming Luo; Haijian Zhou; Xiaoxun Wang; Yu Du; Zhenpeng Li; Jialiang Xu; Baoli Zhu; Xuebin Xu; Biao Kan

doi:10.1128/AAC.02632-16

MCR-1.6, a New MCR Variant Carried by an IncP Plasmid in a Colistin-Resistant Salmonella enterica Serovar Typhimurium Isolate from a Healthy Individual

Antimicrob Agents Chemother. 2017 Apr 24;61(5):e02632-16. doi: 10.1128/AAC.02632-16. Print 2017 May.

Authors

Xin Lu¹, Yongfei Hu², Ming Luo³, Haijian Zhou¹, Xiaoxun Wang^{1

4}, Yu Du¹, Zhenpeng Li¹, Jialiang Xu⁴, Baoli Zhu⁵, Xuebin Xu⁶, Biao Kan⁷

Affiliations

¹ State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China.
² CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
³ Yulin Center for Disease Control and Prevention, Guangxi, China.
⁴ School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, China.
⁵ CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China zhubaoli@im.ac.cn xxb72@sina.com kanbiao@icdc.cn.
⁶ Department of Microbiology, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China zhubaoli@im.ac.cn xxb72@sina.com kanbiao@icdc.cn.
⁷ State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China zhubaoli@im.ac.cn xxb72@sina.com kanbiao@icdc.cn.

Abstract

In this study, we report a novel mcr-1 gene variant, named mcr-1.6, carried by an IncP plasmid in a colistin-resistant Salmonella enterica serovar Typhimurium isolate from a healthy person. Compared with mcr-1, the mcr-1.6 gene contains two single-nucleotide polymorphisms, one of which results in an arginine to histidine variation (Arg536→His). The plasmid carrying the mcr-1.6 gene was designated pMCR1.6_P053 and is similar to a recently discovered mcr-1-bearing plasmid found in Klebsiella pneumoniae.

Keywords: IncP plasmid; MCR-1.6; Salmonella enterica serovar Typhimurium; colistin resistance.

MeSH terms

Amino Acid Substitution / genetics
Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics*
Base Sequence
Colistin / pharmacology*
Conjugation, Genetic / genetics
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics*
Ethanolaminephosphotransferase / genetics*
Female
Humans
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics
Membrane Proteins / genetics
Microbial Sensitivity Tests
Middle Aged
Multilocus Sequence Typing
Plasmids / genetics*
Polymorphism, Single Nucleotide / genetics
Salmonella typhimurium / drug effects
Salmonella typhimurium / genetics*
Salmonella typhimurium / isolation & purification
Sequence Analysis, DNA

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA, Bacterial
Membrane Proteins
Ethanolaminephosphotransferase
Colistin