Quantitation and tissue localization of the cellular retinoic acid-binding protein

J Cell Physiol. 1987 Dec;133(3):482-90. doi: 10.1002/jcp.1041330308.

Abstract

The distribution of the cellular retinoic acid-binding protein (CRABP) in some rat tissues has been determined, and the protein has been localized by immunocytochemical techniques in sections from rat testis. In the testis CRABP was found in the seminiferous tubuli with Sertoli cells and the spermatogonia most intensely stained. All other cells of the germinal epithelium appeared largely devoid of CRABP. By use of an enzyme-linked immunosorbent assay CRABP was quantitatively estimated in several tissues and the highest levels were found in testis and eye. Comparisons of the tissue levels of CRABP and of the cellular retinol-binding protein (CRBP) did not reveal any apparent correlation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Immune Sera / immunology
  • Immunohistochemistry
  • Male
  • Molecular Sequence Data
  • Peptides / chemical synthesis
  • Peptides / immunology
  • Rats
  • Rats, Inbred Strains
  • Receptors, Retinoic Acid
  • Testis / cytology
  • Testis / metabolism
  • Tissue Distribution

Substances

  • Carrier Proteins
  • Immune Sera
  • Peptides
  • Receptors, Retinoic Acid