Physiology and pathogenicity of cpdB deleted mutant of avian pathogenic Escherichia coli

Res Vet Sci. 2017 Apr;111:21-25. doi: 10.1016/j.rvsc.2016.11.010. Epub 2016 Nov 17.

Abstract

Avian colibacillosis is one of the most common infectious diseases caused partially or entirely by avian pathogenic Escherichia coli (APEC) in birds. In addition to spontaneous infection, APEC can also cause secondary infections that result in greater severity of illness and greater losses to the poultry industry. In order to assess the role of 2', 3'-cyclic phosphodiesterase (cpdB) in APEC on disease physiology and pathogenicity, an avian pathogenic Escherichia coli-34 (APEC-34) cpdB mutant was obtained using the Red system. The cpdB mutant grew at a slower rate than the natural strain APEC-34. Scanning electron microscopy (SEM) indicated that the bacteria of the cpdB mutant were significantly longer than the bacteria observed in the natural strain (P<0.01), and that the width of the cpdB mutant was significantly smaller than its natural counterpart (P<0.01). In order to evaluate the role of cpdB in APEC in the colonization of internal organs (lung, liver and spleen) in poultry, seven-day-old SPF chicks were infected with 109CFU/chick of the cpdB mutant or the natural strain. No colonizations of cpdB mutants were observed in the internal organs 10days following the infection, though numerous natural strains were observed at 20days following infection. Additionally, the relative expression of division protein ftsZ, outer membrane protein A ompA, ferric uptake regulator fur and tryptophanase tnaA genes in the mutant strain were all significantly lower than in the natural strain (P<0.05 or P<0.01). These results suggested that cpdB is involved in the long-term colonization of APEC in the internal organs of the test subjects. The deletion of the cpdB gene also significantly affected the APEC growth and morphology.

Keywords: Avian pathogenic Escherichia coli (APEC); Deletion; Pathogenicity; Physiology; cpdB gene.

MeSH terms

  • Animals
  • Chickens / microbiology*
  • Escherichia coli / genetics*
  • Escherichia coli / pathogenicity
  • Escherichia coli / physiology
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Escherichia coli Proteins / genetics*
  • Gene Deletion
  • Gene Expression Regulation, Bacterial / physiology
  • Gene Expression Regulation, Enzymologic / physiology
  • Nucleotidases / genetics
  • Nucleotidases / metabolism*
  • Poultry Diseases / microbiology*
  • Virulence
  • Virulence Factors / genetics

Substances

  • Escherichia coli Proteins
  • Virulence Factors
  • Nucleotidases
  • 3'-nucleotidase