Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells

Int J Biol Macromol. 2017 Jun:99:570-577. doi: 10.1016/j.ijbiomac.2017.03.008. Epub 2017 Mar 4.

Abstract

Seleno-polysaccharides possess a variety of biological activities. In the present study, we further investigated the immunomodulatory effects of low molecular-weight seleno-aminopolysaccharides (LSA) in intestinal porcine epithelial cells (IPEC-1), and the molecular mechanisms of these effects. Analysis by ELISAs revealed that LSA could significantly increase the secretion of nitric oxide (NO), interleukin- 6 (IL-6), interleukin- 10 (IL-10), and tumor necrosis factor alpha (TNF-α). Moreover, LSA dramatically increased the gene expression levels of TNF-α, IL-6, IL-10, and iNOS in IPEC-1 cells, as determined by qRT-PCR. Western blot analysis further determined that LSA promotes inhibitor kappa B α (IĸBα), nuclear factor- kappa B (NF-κB) p65 phosphorylation. Taken together, these findings suggested that LSA has immunomodulatory activity on IPEC-1 cells, and its mechanism may be related to activation of the NF-ĸB signaling pathway.

Keywords: Immunomodulatory activity; Intestinal epithelial cells; Low molecular-weight seleno-aminopolysaccharide.

MeSH terms

  • Adjuvants, Immunologic / chemistry*
  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Cell Line
  • Cell Survival / drug effects
  • Cytokines / metabolism
  • Gene Expression Regulation / drug effects
  • Intestinal Mucosa / cytology*
  • Molecular Weight
  • NF-kappa B / metabolism
  • Nitric Oxide / biosynthesis
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / genetics
  • Polysaccharides / chemistry*
  • Polysaccharides / pharmacology*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Selenium / chemistry*
  • Signal Transduction / drug effects
  • Swine

Substances

  • Adjuvants, Immunologic
  • Cytokines
  • NF-kappa B
  • Polysaccharides
  • RNA, Messenger
  • Nitric Oxide
  • Nitric Oxide Synthase Type II
  • Selenium