"Perfect" designer chromosome V and behavior of a ring derivative

doi:10.1126/science.aaf4704

. 2017 Mar 10;355(6329):eaaf4704.

doi: 10.1126/science.aaf4704.

"Perfect" designer chromosome V and behavior of a ring derivative

Ze-Xiong Xie^{1

2}, Bing-Zhi Li^{1

2}, Leslie A Mitchell³, Yi Wu^{1

2}, Xin Qi^{1

2}, Zhu Jin^{1

2}, Bin Jia^{1

2}, Xia Wang^{1

2}, Bo-Xuan Zeng^{1

2}, Hui-Min Liu^{1

2}, Xiao-Le Wu^{1

2}, Qi Feng^{1

2}, Wen-Zheng Zhang^{1

2}, Wei Liu^{1

2}, Ming-Zhu Ding^{1

2}, Xia Li^{1

2}, Guang-Rong Zhao^{1

2}, Jian-Jun Qiao^{1

2}, Jing-Sheng Cheng^{1

2}, Meng Zhao^{1

2}, Zheng Kuang³, Xuya Wang³, J Andrew Martin³, Giovanni Stracquadanio^{4

5}, Kun Yang⁴, Xue Bai^{1

2}, Juan Zhao^{1

2}, Meng-Long Hu^{1

2}, Qiu-Hui Lin^{1

2}, Wen-Qian Zhang^{1

2}, Ming-Hua Shen^{1

2}, Si Chen^{1

2}, Wan Su^{1

2}, En-Xu Wang^{1

2}, Rui Guo^{1

2}, Fang Zhai^{1

2}, Xue-Jiao Guo^{1

2}, Hao-Xing Du^{1

2}, Jia-Qing Zhu^{1

2}, Tian-Qing Song^{1

2}, Jun-Jun Dai^{1

2}, Fei-Fei Li^{1

2}, Guo-Zhen Jiang^{1

2}, Shi-Lei Han^{1

2}, Shi-Yang Liu^{1

2}, Zhi-Chao Yu^{1

2}, Xiao-Na Yang^{1

2}, Ken Chen^{1

2}, Cheng Hu^{1

2}, Da-Shuai Li^{1

2}, Nan Jia^{1

2}, Yue Liu^{1

2}, Lin-Ting Wang^{1

2}, Su Wang^{1

2}, Xiao-Tong Wei^{1

2}, Mei-Qing Fu^{1

2}, Lan-Meng Qu^{1

2}, Si-Yu Xin^{1

2}, Ting Liu^{1

2}, Kai-Ren Tian^{1

2}, Xue-Nan Li^{1

2}, Jin-Hua Zhang^{1

2}, Li-Xiang Song^{1

2}, Jin-Gui Liu^{1

2}, Jia-Fei Lv^{1

2}, Hang Xu^{1

2}, Ran Tao^{1

2}, Yan Wang^{1

2}, Ting-Ting Zhang^{1

2}, Ye-Xuan Deng^{1

2}, Yi-Ran Wang^{1

2}, Ting Li^{1

2}, Guang-Xin Ye^{1

2}, Xiao-Ran Xu^{1

2}, Zheng-Bao Xia^{1

2}, Wei Zhang^{1

2}, Shi-Lan Yang^{1

2}, Yi-Lin Liu^{1

2}, Wen-Qi Ding^{1

2}, Zhen-Ning Liu^{1

2}, Jun-Qi Zhu^{1

2}, Ning-Zhi Liu^{1

2}, Roy Walker⁶, Yisha Luo⁶, Yun Wang⁷, Yue Shen⁷, Huanming Yang^{7

8}, Yizhi Cai⁶, Ping-Sheng Ma¹, Chun-Ting Zhang¹, Joel S Bader⁴, Jef D Boeke³, Ying-Jin Yuan^{9

2}

Affiliations

¹ Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
² SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
³ Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, Langone Medical Center, New York University, New York City, NY 10016, USA.
⁴ High Throughput Biology Center and Department of Biomedical Engineering, Johns Hopkins University, Baltimore 21205, MD, USA.
⁵ School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, England, UK.
⁶ School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
⁷ BGI-Shenzhen, Shenzhen 518083, PR China.
⁸ James D. Watson Institute of Genome Sciences, Hangzhou 310058, PR China.
⁹ Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. yjyuan@tju.edu.cn.

PMID: 28280151
DOI: 10.1126/science.aaf4704

"Perfect" designer chromosome V and behavior of a ring derivative

Ze-Xiong Xie et al. Science. 2017.

. 2017 Mar 10;355(6329):eaaf4704.

doi: 10.1126/science.aaf4704.

Authors

Affiliations

¹ Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
² SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin 300072, PR China.
³ Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, Langone Medical Center, New York University, New York City, NY 10016, USA.
⁴ High Throughput Biology Center and Department of Biomedical Engineering, Johns Hopkins University, Baltimore 21205, MD, USA.
⁵ School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, England, UK.
⁶ School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK.
⁷ BGI-Shenzhen, Shenzhen 518083, PR China.
⁸ James D. Watson Institute of Genome Sciences, Hangzhou 310058, PR China.
⁹ Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. yjyuan@tju.edu.cn.

PMID: 28280151
DOI: 10.1126/science.aaf4704

Abstract

Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the "Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

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Comment in

Synthetic yeast chromosomes help probe mysteries of evolution.
Maxmen A. Maxmen A. Nature. 2017 Mar 9;543(7645):298-299. doi: 10.1038/nature.2017.21615. Nature. 2017. PMID: 28300123 No abstract available.
Synthetic Biology: Building a custom eukaryotic genome de novo.
Burgess DJ. Burgess DJ. Nat Rev Genet. 2017 Apr 12;18(5):274. doi: 10.1038/nrg.2017.30. Nat Rev Genet. 2017. PMID: 28400600 No abstract available.

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"Perfect" designer chromosome V and behavior of a ring derivative

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"Perfect" designer chromosome V and behavior of a ring derivative

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