The proteomic profile of the acquired enamel pellicle according to its location in the dental arches

Arch Oral Biol. 2017 Jul:79:20-29. doi: 10.1016/j.archoralbio.2017.03.001. Epub 2017 Mar 3.

Abstract

Objective: This study evaluated the variation in the protein profile of the acquired enamel pellicle (AEP) formed in vivo according to its location in the dental arches.

Design: The AEP was formed for 120min in 9 volunteers. Pellicle formed at upper+lower anterior facial (ULAFa; teeth 13-23 and 33-43), upper anterior palatal (UAPa; teeth 13-23), lower anterior lingual (LALi; teeth 33-43), upper+lower posterior facial (ULPFa; teeth 14-17 24-27, 34-37 and 44-47), upper posterior palatal (UPPa; teeth 14-17 and 24-27) and lower posterior lingual (LPLi; teeth 34-37 and 44-47) regions were collected separately and processed for analysis by label-free LC-ESI-MS/MS.

Results: Three-hundred sixty three proteins were identified in total, twenty-five being common to all the locations, such as Protein S100-A8, Lysozyme C, Lactoferrin, Statherin, Ig alpha-2, ALB protein, Myeloperoxidase and SMR3B. Many proteins were found exclusively in the AEP collected from one of the regions (46-UAPa, 33-LALi, 59-ULAFa, 31-ULPFa, 44-LPLi and 39-UPPa).

Conclusions: The protein composition of the AEP varied according to its location in the dental arches. These results provide important insights for understanding the differential protective roles of the AEP as a function of its location in the dental arches.

Keywords: Acquired pellicle; Enamel; Proteomics; Saliva.

MeSH terms

  • Calcium-Binding Proteins / metabolism
  • Calponins
  • Cystatins
  • Dental Arch / metabolism*
  • Dental Pellicle / metabolism*
  • Female
  • Humans
  • Lactoferrin / metabolism
  • Male
  • Microfilament Proteins / metabolism
  • Muramidase / metabolism
  • Peroxidase
  • Proteome / metabolism*
  • Proteomics / methods*
  • S100 Proteins / metabolism
  • Saliva / chemistry*
  • Saliva / metabolism
  • Salivary Proteins and Peptides
  • Serum Albumin, Human / metabolism
  • Tandem Mass Spectrometry / methods
  • Volunteers

Substances

  • ALB protein, human
  • Calcium-Binding Proteins
  • Cystatins
  • Microfilament Proteins
  • Proteome
  • S100 Proteins
  • S100A1 protein
  • STATH protein, human
  • Salivary Proteins and Peptides
  • Peroxidase
  • Muramidase
  • lysozyme C, human
  • Lactoferrin
  • Serum Albumin, Human