Analysis of Antiproliferative Effect of Chamerion Angustifolium Water Extract and Its Fractions on Several Breast Cancer Cell Lines

Adv Med Sci. 2017 Mar;62(1):158-164. doi: 10.1016/j.advms.2016.08.002. Epub 2017 Mar 7.


Purpose: To evaluate the antiproliferative effect of the aerial part of Chamerion angustifolium (L.) Holub. (Onagraceae) extract and its fractions in vitro. This is the first study on the anti-proliferative effect of C. angustifolium on 3 distinct breast cancer cell lines.

Material/methods: Breast cancer cell lines MCF7, MDA-MB-468 and MDA-MB-231 were exposed to different concentrations of the water extract of C. angustifolium, where DPPH radical scavenging activity was 0.018-0.443mg/ml, expressed in rutin equivalents. Cell growth was analyzed after 24, 48 and 72h of incubation. Solid-phase extraction was applied for the fractionation of C. angustifolium water extract and MDA-MB-468 cell line growth was tested using different fractions.

Results: The concentrations corresponding to radical scavenging activity of 0.117 and 0.266mg/ml caused MCF7 cells growth inhibition, while in the samples exposed to the highest concentration (0.355 and 0.443mg/ml) no proliferation was register, suggesting cell death. MDA-MB-468 cell analysis showed similar responses. MDA-MB-231 demonstrated cell growth inhibition following the exposure to all analyzed high extract doses (0.117-0.443mg/ml). MDA-MB-468 cells were selected to evaluate the effect of fractions. In the samples exposed to the fraction containing the highest amount (91%) of oenothein B, at the concentration of 0.117mg/ml a pronounced cell growth inhibition while at higher concentrations (0.266 and 0.443mg/ml) no cell proliferation was observed.

Conclusions: The consumption of C. angustifolium herb can be advantageous, alongside with conventional breast cancer treatment.

Keywords: Fractionation; MCF7 cells; MDA-MB-231 cells; MDA-MB-468 cells; Oenothein B.

MeSH terms

  • Apoptosis / drug effects
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / pathology*
  • Cell Proliferation / drug effects*
  • Female
  • Flavonoids / pharmacology*
  • Humans
  • Onagraceae / chemistry*
  • Phytotherapy*
  • Plant Extracts / pharmacology*
  • Tumor Cells, Cultured


  • Flavonoids
  • Plant Extracts