Proteome-Scale Human Interactomics

Trends Biochem Sci. 2017 May;42(5):342-354. doi: 10.1016/j.tibs.2017.02.006. Epub 2017 Mar 8.


Cellular functions are mediated by complex interactome networks of physical, biochemical, and functional interactions between DNA sequences, RNA molecules, proteins, lipids, and small metabolites. A thorough understanding of cellular organization requires accurate and relatively complete models of interactome networks at proteome scale. The recent publication of four human protein-protein interaction (PPI) maps represents a technological breakthrough and an unprecedented resource for the scientific community, heralding a new era of proteome-scale human interactomics. Our knowledge gained from these and complementary studies provides fresh insights into the opportunities and challenges when analyzing systematically generated interactome data, defines a clear roadmap towards the generation of a first reference interactome, and reveals new perspectives on the organization of cellular life.

Publication types

  • Review
  • Research Support, N.I.H., Extramural

MeSH terms

  • Humans
  • Protein Binding
  • Protein Interaction Mapping*
  • Protein Interaction Maps*
  • Proteins / chemistry
  • Proteins / metabolism*
  • Proteome / metabolism*
  • Proteomics


  • Proteins
  • Proteome