Bioengineered 3D Models for Studying Human Cell-Tuberculosis Interactions

Sulayman Benmerzoug; Valerie F J Quesniaux

doi:10.1016/j.tim.2017.02.009

Bioengineered 3D Models for Studying Human Cell-Tuberculosis Interactions

Trends Microbiol. 2017 Apr;25(4):245-246. doi: 10.1016/j.tim.2017.02.009. Epub 2017 Mar 8.

Authors

Sulayman Benmerzoug¹, Valerie F J Quesniaux²

Affiliations

¹ CNRS, UMR 7355, Orleans, France; University of Orleans, INEM, Experimental and Molecular Immunology and Neurogenetics, Orleans, France.
² CNRS, UMR 7355, Orleans, France; University of Orleans, INEM, Experimental and Molecular Immunology and Neurogenetics, Orleans, France. Electronic address: quesniaux@cnrs-orleans.fr.

PMID: 28284875
DOI: 10.1016/j.tim.2017.02.009

Abstract

In vivo animal models have intrinsic limitations for studying relationships between tuberculosis and its host and there is a need for alternative, in vitro cellular models. A microsphere-based 3D in vitro culture system of Mycobacterium tuberculosis-infected human blood mononuclear cells was reported to address specific aspects of host-pathogen interactions.

Keywords: Tuberculosis; bioelectrospray; extracellular matrix; granuloma; in vitro 3D cell model; microsphere.

Publication types

Comment

MeSH terms

Animals
CD4-Positive T-Lymphocytes / immunology*
Cell Communication / immunology*
Cell Culture Techniques / methods
Cells, Cultured
Host-Pathogen Interactions / immunology*
Humans
Macrophages / immunology*
Mice
Microspheres
Mycobacterium tuberculosis / immunology*
Mycobacterium tuberculosis / pathogenicity
Tuberculoma / microbiology
Tuberculoma / pathology
Tuberculosis, Pulmonary / immunology*
Tuberculosis, Pulmonary / microbiology
Tuberculosis, Pulmonary / pathology
Tumor Necrosis Factor-alpha / immunology

Substances

Tumor Necrosis Factor-alpha