Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle

Nat Commun. 2017 Mar 13;8:14664. doi: 10.1038/ncomms14664.

Abstract

Upon acquirement of pulmonary circulation, the ancestral heart may have been remodelled coincidently with, or accompanied by, the production and rearrangement of progenitor cells. However, the progenitor populations that give rise to the left ventricle (LV) and sinus venosus (SV) are still ambiguous. Here we show that the expression of Secreted frizzled-related protein Sfrp5 in the mouse identifies common progenitors for the outflow tract (OFT), LV, atrium and SV but not the right ventricle (RV). Sfrp5 expression begins at the lateral sides of the cardiac crescent, excluding early differentiating regions, and continues in the venous pole, which gives rise to the SV. Lineage-tracing analysis revealed that descendants of Sfrp5-expressing cells at E7.5 contribute not only to the SV but also to the LV, atria and OFT and are found also in the dorsal splanchnic mesoderm accompanied by the expression of the secondary heart field marker, Islet1. These findings provide insight into the arrangement of cardiac progenitors for systemic circulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Body Patterning / genetics
  • Cell Lineage / genetics
  • Cell Tracking / methods
  • Coronary Sinus / cytology
  • Coronary Sinus / growth & development
  • Coronary Sinus / metabolism*
  • Embryo, Mammalian
  • Fibroblast Growth Factor 10 / genetics
  • Fibroblast Growth Factor 10 / metabolism
  • Gene Expression
  • Heart Atria / cytology
  • Heart Atria / growth & development
  • Heart Atria / metabolism*
  • Heart Ventricles / cytology
  • Heart Ventricles / growth & development
  • Heart Ventricles / metabolism*
  • Intercellular Signaling Peptides and Proteins / genetics*
  • Intercellular Signaling Peptides and Proteins / metabolism
  • LIM-Homeodomain Proteins / genetics
  • LIM-Homeodomain Proteins / metabolism
  • MEF2 Transcription Factors / genetics
  • MEF2 Transcription Factors / metabolism
  • Mesoderm / cytology
  • Mesoderm / growth & development
  • Mesoderm / metabolism
  • Mice
  • Mice, Transgenic
  • Myocardium / cytology
  • Myocardium / metabolism*
  • Stem Cells / cytology
  • Stem Cells / metabolism*
  • T-Box Domain Proteins / genetics
  • T-Box Domain Proteins / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Biomarkers
  • Fgf10 protein, mouse
  • Fibroblast Growth Factor 10
  • Intercellular Signaling Peptides and Proteins
  • LIM-Homeodomain Proteins
  • MEF2 Transcription Factors
  • Mef2c protein, mouse
  • Sfrp5 protein, mouse
  • T-Box Domain Proteins
  • Tbx1 protein, mouse
  • Transcription Factors
  • insulin gene enhancer binding protein Isl-1