Peptides from gluten digestion: A comparison between old and modern wheat varieties

Food Res Int. 2017 Jan:91:92-102. doi: 10.1016/j.foodres.2016.11.034. Epub 2016 Dec 2.


Coeliac disease is an autoimmune enteropathy that develops in genetically predisposed subjects after the ingestion of gluten or related proteins. Coeliac disease has an increasing incidence in the last years in western countries and it has been suggested that wheat breeding might have contributed to select more toxic forms of gluten. In this work, we analysed gluten peptides generated by in vitro digestion of different old and modern Triticum varieties, using LC-MS. We concluded that old varieties analysed produced a higher quantity of peptides containing immunogenic and toxic sequences than modern ones. Thus old wheat lines are not to be considered "safer" for subjects that are genetically predisposed to celiac disease.

Keywords: Coeliac disease; Gluten peptides; In vitro digestion; Old wheat; Wheat breeding.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Celiac Disease / diagnosis
  • Celiac Disease / genetics
  • Celiac Disease / immunology*
  • Digestion*
  • Genetic Predisposition to Disease
  • Glutens / adverse effects
  • Glutens / immunology
  • Glutens / metabolism*
  • Humans
  • Immunodominant Epitopes
  • Peptide Fragments / adverse effects
  • Peptide Fragments / immunology
  • Peptide Fragments / metabolism*
  • Peptide Mapping / methods
  • Risk Assessment
  • Risk Factors
  • Triticum / adverse effects
  • Triticum / classification
  • Triticum / immunology
  • Triticum / metabolism*


  • Immunodominant Epitopes
  • Peptide Fragments
  • Glutens