L-myo-inositol 1,4,5,6-tetrakisphosphate (3-hydroxy)kinase

Biochem J. 1988 Jan 1;249(1):283-92. doi: 10.1042/bj2490283.


Homogenates of primary-cultured murine bone macrophages contain an enzyme capable of synthesizing myo-[3H]inositol pentakisphosphate from myo-[3H]inositol tetrakisphosphate fractions derived from myo-[3H]inositol-labelled mouse macrophages and chick erythrocytes. D-myo-inositol 1,3,4,5-tetrakis[32P]-phosphate present in the same incubations was not phosphorylated. Since the myo-[3H]inositol-labelled tetrakisphosphate fractions used as substrates consist of a mixture of L-myo-inositol 1,4,5,6-tetrakisphosphate (60-85%) and a periodate-resistant tetrakisphosphate(s) whose characteristics are consistent with those of D-myo-inositol 1,3,4,5-tetrakisphosphate (the preceding paper [Stephens, Hawkins, Carter, Chahwala, Morris, Whetton & Downes (1988) Biochem. J. 249, 271-282] ), these data suggest the existence of a kinase that phosphorylates L-myo-inositol 1,4,5,6-tetrakisphosphate to give a myo-inositol pentakisphosphate. A similar activity was identified in homogenates of rat cerebrum, liver, heart and parotid gland. D-myo-Inositol 1,3,4,5-tetrakis[32P]phosphate in the same incubations was not a substrate. The activity was almost entirely soluble in all the tissues investigated and was found at its greatest specific activity in brain cytosol. The activity was purified 120-fold from a rat brain homogenate by (NH4)2SO4 fractionation and anion-exchange chromatography. The activity was clearly distinct from D-myo-inositol 1,4,5-trisphosphate (3-hydroxy)kinase. Incubation of this partially purified preparation with L-myo-[3H]inositol 1,4,5,6-tetrakisphosphate from chick erythrocytes and [gamma-32P]ATP resulted in the formation of L-myo-[3H]-inositol [1-32P]1,3,4,5,6-pentakisphosphate. The enzyme is therefore identified as an L-myo-inositol 1,4,5,6-tetrakisphosphate (3-hydroxy)kinase.

MeSH terms

  • Animals
  • Brain / enzymology
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Cytosol / enzymology
  • Erythrocytes / enzymology
  • Inositol Phosphates / biosynthesis*
  • Inositol Phosphates / metabolism
  • Macrophages / enzymology
  • Mice
  • Phosphotransferases (Alcohol Group Acceptor)*
  • Phosphotransferases / isolation & purification
  • Phosphotransferases / metabolism*
  • Rats
  • Subcellular Fractions / enzymology
  • Sugar Phosphates / biosynthesis*
  • Tissue Distribution


  • Inositol Phosphates
  • Sugar Phosphates
  • inositol-1,3,4,5-tetrakisphosphate
  • inositol-1,4,5,6-tetrakisphosphate
  • inositol pentaphosphate
  • Phosphotransferases
  • Phosphotransferases (Alcohol Group Acceptor)
  • inositol-tetrakisphosphate 3-kinase