The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 trans-activator

Cell. 1988 Jan 29;52(2):169-78. doi: 10.1016/0092-8674(88)90505-3.


The yeast trans-activator protein GAL4, when expressed in HeLa cells, stimulates transcription from several class B (II) eukaryotic promoters containing GAL4 binding sites either as the full UASG or as synthetic 17-mers. The characteristics of this activation are indistinguishable from those of the SV40 enhancer. Transcription was similarly stimulated from either complex promoter regions containing multiple upstream elements or from a simple promoter region composed of only a TATA box. Addition of a 17-mer GAL4 binding site to the SV40 enhancer resulted in a synergistic enhancement of transcription in the presence of GAL4. Furthermore, chimeras of the human estrogen receptor DNA binding domain and either GAL4 or GCN4 activating "acidic" regions can activate a promoter region controlled by an estrogen-responsive enhancer. Together, these data indicate that the molecular mechanisms responsible for transcriptional enhancement have been conserved from yeast to man.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA, Fungal / genetics*
  • DNA, Recombinant
  • DNA-Binding Proteins
  • Enhancer Elements, Genetic*
  • Estrogens / pharmacology
  • Fungal Proteins / genetics*
  • Globins / genetics
  • HeLa Cells / metabolism
  • Humans
  • Plasmids
  • Promoter Regions, Genetic
  • Rabbits
  • Receptors, Estrogen / genetics
  • Regulatory Sequences, Nucleic Acid*
  • Saccharomyces cerevisiae Proteins*
  • Simian virus 40 / genetics
  • Simplexvirus / enzymology
  • Simplexvirus / genetics
  • Thymidine / genetics
  • Transcription Factors*
  • Transcription, Genetic*
  • Transfection


  • DNA, Fungal
  • DNA, Recombinant
  • DNA-Binding Proteins
  • Estrogens
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Receptors, Estrogen
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Globins
  • Thymidine