Protein conformation in pure and hydrated deep eutectic solvents

A Sanchez-Fernandez; K J Edler; T Arnold; D Alba Venero; A J Jackson

doi:10.1039/c7cp00459a

Protein conformation in pure and hydrated deep eutectic solvents

Phys Chem Chem Phys. 2017 Mar 29;19(13):8667-8670. doi: 10.1039/c7cp00459a.

Authors

A Sanchez-Fernandez¹, K J Edler², T Arnold³, D Alba Venero⁴, A J Jackson⁵

Affiliations

¹ Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK. a.sanchez.fernandez@bath.ac.uk and European Spallation Source, 221 00, Lund, Sweden.
² Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK. a.sanchez.fernandez@bath.ac.uk.
³ Diamond Light Source, Harwell Campus, Didcot, OX11 0DE, UK.
⁴ ISIS Spallation Neutron Source, Harwell Campus, Didcot, OX11 OQX, UK.
⁵ European Spallation Source, 221 00, Lund, Sweden and Department of Physical Chemistry, Lund University, SE-221 00, Lund, Sweden.

PMID: 28300267
DOI: 10.1039/c7cp00459a

Abstract

Deep eutectic solvents (DES) have recently been postulated as possible environments where protein structure may be preserved in the absence of water. Here we present our results towards understanding protein conformation in choline chloride-based DES and mixtures with water. Lysozyme and bovine serum albumin have been investigated by means of circular dichroism and small-angle neutron scattering.