A new type of functional VIP receptor has an affinity for helodermin in human SUP-T1 lymphoblasts

FEBS Lett. 1988 Feb 15;228(2):351-5. doi: 10.1016/0014-5793(88)80030-9.


A new type of VIP receptor was characterized in human SUP-T1 lymphoblasts. The order of potency of unlabeled peptides, in the presence of [125I]helodermin, was: helodermin(1-35)-NH2 = helodermin(1-27)-NH2 greater than helospectin greater than VIP = PHI greater than [D-Ser2]VIP greater than [D-Asp3]VIP greater than [D-His1]VIP greater than or equal to [D-Ala4]VIP greater than or equal to secretin = GRF. This specificity was distinct from that of all VIP receptors described so far in that: (i) the affinity for helodermin (Kd = 3 nM) was higher than that of VIP (Kd = 15 nM) and PHI (Kd = 20 nM); and (ii) position 4 played an important role in ligand binding. The labeled sites were likely to be functional receptors as adenylate cyclase in crude lymphoblastic membranes (200-10,000 x g pellets) was stimulated by peptides, in the presence of GTP, with the following order of potency: helodermin(1-35)-NH2 greater than helodermin(1-27)-NH2 greater than helospectin = VIP = PHI.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Cell Membrane / metabolism
  • Guanosine Triphosphate / pharmacology
  • Humans
  • Intercellular Signaling Peptides and Proteins
  • Kinetics
  • Lymphoma / metabolism*
  • Peptide Fragments / metabolism
  • Peptide PHI / metabolism
  • Peptide PHI / pharmacology
  • Peptides / metabolism*
  • Peptides / pharmacology
  • Receptors, Gastrointestinal Hormone / metabolism*
  • Receptors, Vasoactive Intestinal Peptide
  • T-Lymphocytes / metabolism
  • Tumor Cells, Cultured
  • Vasoactive Intestinal Peptide / metabolism
  • Vasoactive Intestinal Peptide / pharmacology


  • Intercellular Signaling Peptides and Proteins
  • Peptide Fragments
  • Peptide PHI
  • Peptides
  • Receptors, Gastrointestinal Hormone
  • Receptors, Vasoactive Intestinal Peptide
  • Vasoactive Intestinal Peptide
  • Guanosine Triphosphate
  • heliodermin
  • helospectin I
  • helospectin II
  • Adenylyl Cyclases