Objective: To screen out retinoblastoma (RB)-related serum tumor markers by measuring the levels of serum alpha fetoprotein (AFP), carcino-embryonic antigen (CEA), neuron-specific enolase (NSE), carbohydrate antigen 125 (CA125), carbohydrate antigen 153 (CA153), carbohydrate antigen 199 (CA199), and carbohydrate antigen 724 (CA724) in children with RB.
Methods: The levels of seven serum tumor markers (AFP, CEA, NSE, CA125, CA153, CA199, and CA724) were determined in 20 children with RB and 20 healthy children (control) using a chemiluminescent immunoassay.
Results: The serum levels and positive rates of NSE, CA153, and CA199 in the RB group were significantly higher than those in the control group (P<0.05). However, there were no significant differences in the levels of AFP, CEA, CA125, and CA724 between the two groups (P>0.05). NSE had the highest sensitivity, but a relatively low specificity for the diagnosis of RB. CA153 and CA199 had a relatively high specificity, but a relatively low sensitivity for the diagnosis of RB.
Conclusions: The serum levels and positive rates of NSE, CA153, and CA199 are high in children with RB. Combined measurement of these three serum tumor markers may have an important diagnostic value for RB.
目的: 研究7个常用血清肿瘤标志物（TM）在视网膜母细胞瘤（RB）患者的表达，以期筛选出与RB相关的血清肿瘤标记物。
方法: 采用化学发光法检测20例RB患者及20例健康体检者（对照组）的甲胎蛋白（AFP）、癌胚抗原（CEA）、神经元特异性烯醇化酶（NSE）、糖类抗原125（CA125）以及CA153、CA199、CA724的水平。
结果: RB组的NSE、CA153、CA199水平及阳性率均高于对照组（P < 0.05），两组间AFP、CEA、CA125、CA724水平的差异无统计学意义（P > 0.05）。NSE灵敏度最高，但特异度相对较低；CA153、CA199特异度较高，但灵敏度相对较低。
结论: NSE、CA153、CA199在RB组的表达水平及阳性率均较高，三者结合可能可作为视网膜母细胞瘤诊断的标志物。