v-erbA specifically suppresses transcription of the avian erythrocyte anion transporter (band 3) gene

Cell. 1988 Jan 15;52(1):107-19. doi: 10.1016/0092-8674(88)90535-1.


Previous work has established that the v-erbA oncogene inhibits the temperature-induced differentiation of chick erythroblasts transformed with temperature-sensitive oncogene mutants. Here we demonstrate that v-erbA in differentiating erythroblasts specifically arrests expression of the erythrocyte anion transporter (band 3) gene at the transcriptional level. The v-erbA-induced differentiation block can be overcome by inducing cells to differentiate at alkaline pH. Under these conditions, which possibly impair biological activity of v-erbA, the maturing cells now express the anion transporter gene at high levels. However, its transcription is specifically and rapidly suppressed if v-erbA activity is restored by culturing the cells at neutral pH. Similar but less pronounced inhibition of gene expression by v-erbA was observed for the delta-amino-levulinic acid synthase gene. Additional evidence obtained with an inhibitor of band 3 activity suggests that the v-erbA-induced inhibition of band 3 gene expression is at least partly responsible for the differentiation block caused by this oncogene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 5-Aminolevulinate Synthetase / genetics
  • Alpharetrovirus / genetics*
  • Animals
  • Anion Transport Proteins
  • Avian Leukosis Virus / genetics*
  • Carrier Proteins / genetics*
  • Cell Transformation, Viral
  • Cells, Cultured
  • Chick Embryo
  • Chickens
  • Clone Cells
  • Culture Media
  • Cytoskeletal Proteins / biosynthesis
  • Cytoskeletal Proteins / genetics
  • Erythroblasts / metabolism
  • Erythroblasts / microbiology*
  • Fibroblasts
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Globins / biosynthesis
  • Globins / genetics
  • Hemoglobins / biosynthesis
  • Hemoglobins / genetics
  • Hydrogen-Ion Concentration
  • Immunoassay
  • Mutation
  • Oncogenes*
  • RNA, Messenger / biosynthesis
  • Spectrin / biosynthesis
  • Spectrin / genetics
  • Suppression, Genetic
  • Temperature
  • Transcription, Genetic*


  • Anion Transport Proteins
  • Carrier Proteins
  • Culture Media
  • Cytoskeletal Proteins
  • Hemoglobins
  • RNA, Messenger
  • Spectrin
  • Globins
  • 5-Aminolevulinate Synthetase