DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity

Håvard Foyn; Paul R Thompson; Thomas Arnesen

doi:10.1007/978-1-4939-6850-3_2

DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity

Methods Mol Biol. 2017:1574:9-15. doi: 10.1007/978-1-4939-6850-3_2.

Authors

Håvard Foyn¹, Paul R Thompson², Thomas Arnesen^{3

4}

Affiliations

¹ Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5020, Bergen, Norway.
² Department of Biochemistry and Molecular Pharmacology, UMASS Medical School, 365 Plantation Street, Worcester, MA, 01605, USA.
³ Department of Molecular Biology, University of Bergen, Thormøhlensgate 55, N-5020, Bergen, Norway. Thomas.Arnesen@uib.no.
⁴ Department of Surgery, Haukeland University Hospital, N-5021, Bergen, Norway. Thomas.Arnesen@uib.no.

PMID: 28315240
DOI: 10.1007/978-1-4939-6850-3_2

Abstract

We here describe a quick and easy method to quantitatively measure in vitro acetylation activity of not only N-terminal acetyltransferase (NAT) enzymes, but acetyltransferases using acetyl-coenzyme A as an acetyl donor in general.

Keywords: 5,5′-dithiobis-(2-nitrobenzoic acid); Ac-CoA; Acetyltransferase; Enzyme activity; Enzyme assay; N-terminal acetyltransferase (NAT); Thiol detection; Thiol quantification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyl Coenzyme A / chemistry
Acetyl Coenzyme A / metabolism
Acetylation
Dithionitrobenzoic Acid / chemistry*
Enzyme Activation
Enzyme Assays / methods*
In Vitro Techniques
N-Terminal Acetyltransferases / chemistry*
N-Terminal Acetyltransferases / metabolism*
Substrate Specificity

Substances

Acetyl Coenzyme A
Dithionitrobenzoic Acid
N-Terminal Acetyltransferases