Carbon quantum dots as fluorescence resonance energy transfer sensors for organophosphate pesticides determination

Xiaoli Wu; Yang Song; Xu Yan; Chengzhou Zhu; Yongqiang Ma; Dan Du; Yuehe Lin

doi:10.1016/j.bios.2017.03.010

Carbon quantum dots as fluorescence resonance energy transfer sensors for organophosphate pesticides determination

Biosens Bioelectron. 2017 Aug 15:94:292-297. doi: 10.1016/j.bios.2017.03.010. Epub 2017 Mar 7.

Authors

Xiaoli Wu¹, Yang Song², Xu Yan², Chengzhou Zhu², Yongqiang Ma³, Dan Du², Yuehe Lin⁴

Affiliations

¹ School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States; College of Science, China Agricultural University, Beijing 100193, China.
² School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States.
³ College of Science, China Agricultural University, Beijing 100193, China.
⁴ School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States. Electronic address: annie.du@wsu.edu.

PMID: 28315592
DOI: 10.1016/j.bios.2017.03.010

Abstract

Carbon quantum dots (CQDs) obtained from natural organics attract significant attention due to the abundance of carbon sources, varieties of heteroatom doping (such as N, S, P) and good biocompatibility of precursor. In this study, tunable fluorescence emission CQDs originated from chlorophyll were synthesized and characterized. The fluorescence emission can be effectively quenched by gold nanoparticles (Au NPs) via fluorescence resonance energy transfer (FRET). Thiocholine, which was produced from acetylthiocholine (ATC) by the hydrolysis of butyrylcholinesterase (BChE), could cause the aggregation of Au NPs and the corresponding recovery of FRET-quenched fluorescence emission. The catalytic activity of BChE could be irreversibly inhibited by organophosphorus pesticides (OPs), thus, the recovery effect was reduced. By evaluating the fluorescence emission intensity of CQDs, a FRET-based sensing platform for OPs determination was established. Paraoxon was studied as an example of OPs. The sensing platform displayed a linear relationship with the logarithm of the paraoxon concentrations in the range of 0.05-50μgL^-1 and the limit of detection (LOD) was 0.05μgL^-1. Real sample study in tap and river water revealed that this sensing platform was repeatable and accurate. The results indicate that the OP sensor is promising for applications in food safety and environmental monitoring.

Keywords: Carbon quantum dots; Fluorescence resonance energy transfer; Fluorescent sensor; Gold nanoparticles; Organophosphate pesticide.

MeSH terms

Biosensing Techniques / methods*
Carbon
Fluorescence
Fluorescence Resonance Energy Transfer / methods*
Humans
Metal Nanoparticles / chemistry
Organophosphates / chemistry
Organophosphates / isolation & purification*
Pesticides / chemistry
Pesticides / isolation & purification*
Quantum Dots / chemistry

Substances

Organophosphates
Pesticides
Carbon