Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein

EMBO J. 1987 Dec 20;6(13):4143-8.

Abstract

Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinin / genetics*
  • DNA Restriction Enzymes
  • Dictyostelium / genetics*
  • Genes*
  • Genes, Fungal*
  • Genetic Vectors
  • RNA, Messenger / genetics*
  • Recombination, Genetic*
  • Transformation, Genetic

Substances

  • RNA, Messenger
  • Actinin
  • DNA Restriction Enzymes