Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA

Methods Mol Biol. 2017;1587:63-69. doi: 10.1007/978-1-4939-6892-3_6.

Abstract

Both telomere length and telomere G-tail length are altered in human diseases such as cancer and age-related diseases. While most methods for measurement of G-tail and telomere length require electrophoresis, centrifugation, radioisotope labeling and autoradiography, G-tail telomere HPA provides a convenient and useful tool for the examination of G-tail length with a high-throughput platform using genomic DNA or cell lysate. G-tail telomere HPA may be applicable for clinical diagnostics as well as drug target screening.

Keywords: Aging; Cancer; G-Tail; HPA; hybridization protection assay; High-throughput; Risk assessment.

MeSH terms

  • DNA / genetics
  • Humans
  • Telomerase / genetics
  • Telomere / genetics*

Substances

  • DNA
  • Telomerase