Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening

Methods Mol Biol. 2017;1563:269-280. doi: 10.1007/978-1-4939-6810-7_17.

Abstract

Applying the right acquisition method in a fluorescence imaging-based screening context is of great importance to obtain an appropriate readout and to select the right scale of the screen. In order to save imaging time and data, we have developed routines for multiscale targeted imaging, providing both a broad overview of a sample and additional in-depth information for targets of interest identified within the screen. These objects can be identified and acquired on-the-fly by an interconnection of image acquisition and image analysis.

Keywords: Automated imaging; High content screening; High-throughput screening; KNIME; Targeted imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers
  • Cell Line
  • Genes, Reporter
  • High-Throughput Screening Assays / methods*
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Fluorescence / methods*
  • Phenotype*
  • Software
  • Workflow

Substances

  • Biomarkers