A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography

M C Peitsch; T J Kovacsovics; H Isliker

doi:10.1016/0022-1759(88)90428-0

A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using fast protein chromatography

J Immunol Methods. 1988 Apr 6;108(1-2):265-9. doi: 10.1016/0022-1759(88)90428-0.

Authors

M C Peitsch¹, T J Kovacsovics, H Isliker

Affiliation

¹ Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.

PMID: 2832479
DOI: 10.1016/0022-1759(88)90428-0

Abstract

The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid / methods*
Complement Activating Enzymes / immunology
Complement Activating Enzymes / isolation & purification*
Complement Activating Enzymes / metabolism
Complement C1 / immunology
Complement C1 / isolation & purification*
Complement C1 / metabolism
Complement C1r
Complement C1s / immunology
Complement C1s / isolation & purification*
Complement C1s / metabolism
Complement Pathway, Classical
Enzyme Activation
Enzyme Precursors / immunology
Enzyme Precursors / isolation & purification*
Enzyme Precursors / metabolism
Humans

Substances

Complement C1
Enzyme Precursors
Complement Activating Enzymes
Complement C1r
Complement C1s