Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo

J Immunol Res. 2017:2017:4645701. doi: 10.1155/2017/4645701. Epub 2017 Feb 23.

Abstract

Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus (equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract exhibited evident microbicide activity against S. aureus with P. aeruginosa but it was substantially strengthened in K. pinnata + CecP1 extract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization even better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide activity of CecP1 accelerating elimination of bacteria.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Infective Agents / therapeutic use*
  • Cefazolin / therapeutic use
  • Humans
  • Immunomodulation
  • Kalanchoe / genetics*
  • Male
  • Peptides / genetics
  • Peptides / therapeutic use*
  • Plant Extracts / therapeutic use*
  • Plants, Genetically Modified
  • Pseudomonas Infections / drug therapy*
  • Pseudomonas aeruginosa / physiology*
  • Rats
  • Rats, Wistar
  • Recombinant Proteins / genetics
  • Recombinant Proteins / therapeutic use*
  • Staphylococcal Infections / drug therapy*
  • Staphylococcus aureus / physiology*
  • Swine
  • Wound Healing / drug effects
  • Wound Infection / drug therapy*

Substances

  • Anti-Infective Agents
  • Peptides
  • Plant Extracts
  • Recombinant Proteins
  • Cefazolin
  • cecropin P1-LI