Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil

Sharjeel Abid; Zulfiqar Ali Raza; Tanveer Hussain

doi:10.1007/s13205-016-0452-4

Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil

3 Biotech. 2016 Dec;6(2):142. doi: 10.1007/s13205-016-0452-4. Epub 2016 Jun 23.

Authors

Sharjeel Abid¹, Zulfiqar Ali Raza², Tanveer Hussain¹

Affiliations

¹ Chemistry Research Laboratory, National Textile University, Faisalabad, 37610, Pakistan.
² Chemistry Research Laboratory, National Textile University, Faisalabad, 37610, Pakistan. zarazapk@yahoo.com.

Abstract

The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy. The ESI-MS analysis exhibited the major polyhydroxybutyrate with a molecular mass of m/z 448.5.

Keywords: PHAs; Polyhydroxyalkanoates; Pseudomonas aeruginosa; Shake flask; Soybean oil.